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Agilent 1100/1200 HPLC ChemStation Operation Course Number H4033A Laboratory Manual

Lab Exercise: Introduction to the HPLC ChemStation In this Laboratory You Will: 2 In this Laboratory You Will: • Access the HPLC ChemStation software

Lab Exercise: Quantification Integration 92 9) Check the integration for signal B as well. Make certain that both signals are mapped to the DAD Def

Lab Exercise: Quantification Setting up Signal Details 93 Setting up Signal Details The Signal Details dialog box describes which signals will be ev

Lab Exercise: Quantification Building a Calibration Table 94 Building a Calibration Table You have already loaded and integrated the lowest level st

Lab Exercise: Quantification Building a Calibration Table 95 12) Now find the last major peak at approximately 3.3 minutes. The compound name is o-t

Lab Exercise: Quantification Setting up Low and High Amount Limits for Calibration Standards 96 Setting up Low and High Amount Limits for Calibration

Lab Exercise: Quantification Setting up Qualifiers (Diode Array only) 97 Setting up Qualifiers (Diode Array only) A qualifier peak is used to confir

Lab Exercise: Quantification Testing the Calibration Table 98 Testing the Calibration Table 1) Load the medium standard's data file, med.d. G

Lab Exercise: Running a Sequence

Lab Exercise: Running a Sequence In this Laboratory You Will: 100 In this Laboratory You Will: • Build a sequence with automated recalibration of t

Lab Exercise: Running a Sequence Building a Sequence 101 Building a Sequence 1) Go to the Method and Run Control view. 2) Under the Sequence menu,

Lab Exercise: Introduction to the HPLC ChemStation Accessing the HPLC ChemStation 3 Accessing the HPLC ChemStation 1) Find the Start button in the b

Lab Exercise: Running a Sequence Building a Sequence 102 7) Now enter the Sequence Table from the Sequence menu or from the sequence tray setup too

Lab Exercise: Running a Sequence Building a Sequence 103 8) OK the Sequence Table after completing the entries and save the sequence either through

Lab Exercise: Running a Sequence Sequence Summary Reports 104 Sequence Summary Reports The ChemStation can print a variety of standard reports for i

Lab Exercise: Running a Sequence Starting the Sequence 105 Starting the Sequence 1) Make certain that Quant.M is currently loaded. 2) View the Run

Lab Exercise: Running a Sequence Pausing a Sequence 106 Pausing a Sequence 1) Pausing a sequence to run a priority sample is quite easily accomplis

Lab Exercise: Running a Sequence Post Sequence 107 Post Sequence 1) Examine the printed reports. How did your sequence go? 2) If you have errors

Lab Exercise: Running a Sequence Post Sequence 108

Lab Exercise: Sequence Review and Reprocessing

Lab Exercise: Sequence Review and Reprocessing In this Laboratory You Will: 110 In this Laboratory You Will: • Load a sequence into the sequence na

Lab Exercise: Sequence Review and Reprocessing Reprocess with Sequence Folder Method 111 Reprocess with Sequence Folder Method You will reprocess a s

Lab Exercise: Introduction to the HPLC ChemStation Accessing the HPLC ChemStation 4 6) You can also change views by utilizing the Navigation Buttons.

Lab Exercise: Sequence Review and Reprocessing Reprocess with Sequence Folder Method 112 You will update the Sequence Folder Method with new reten

Lab Exercise: Sequence Review and Reprocessing Reprocess with Sequence Folder Method 113 4) First, let’s change the retention time windows so

Lab Exercise: Sequence Review and Reprocessing Reprocess with Sequence Folder Method 114 7) Now, take a look at the calibration curve. Select the

Lab Exercise: Sequence Review and Reprocessing Reprocess with Sequence Folder Method 115 The Sequence folder .S file is currently loaded. If you wan

Lab Exercise: Sequence Review and Reprocessing Reprocess with Sequence Folder Method 116 16) To reprocess the sequence, select the Start sequence

Lab Exercise: Sequence Review and Reprocessing Reprocess with DA Methods 117 Reprocess with DA Methods In this section, you will see how to change t

Lab Exercise: Sequence Review and Reprocessing Reprocess with DA Methods 118 4) You can review the data to check integration by stepping through the

Lab Exercise: Sequence Review and Reprocessing Reprocess with DA Methods 119 12) Resave the DA method. Make certain you have saved the desired ch

Lab Exercise: Sequence Review and Reprocessing Reprocess with DA Methods 120 19) Now, reprocess the sequence. Take a look at the resulting Compound

Lab Exercise: Sequence Review and Reprocessing Batch Review 121 Batch Review If you generate a large number of samples on a daily basis and would li

Lab Exercise: Introduction to the HPLC ChemStation Configuration Editor 5 Configuration Editor You customer engineer configured your instrument during

Lab Exercise: Sequence Review and Reprocessing Batch Review 122

Lab Exercise: Advanced Reporting

Lab Exercise: Advanced Reporting In this Laboratory You Will: 124 In this Laboratory You Will: Preview the following report styles: • GLP • Perfor

Lab Exercise: Advanced Reporting Adding a Report Header 125 Adding a Report Header You may add an individualized header to many of the report styles

Lab Exercise: Advanced Reporting Adding a Report Header 126 Note: The GLP report style is not in any way connected to the Save GLP data selection f

Lab Exercise: Advanced Reporting Performance Reports (System Suitability) 127 Performance Reports (System Suitability) Many chromatographers utilize

Lab Exercise: Advanced Reporting Performance Reports (System Suitability) 128 Select System Suitability from the Report menu, followed by Edit Noise

Lab Exercise: Advanced Reporting Automated Peak Purity 129 Automated Peak Purity The Short + Spectrum, Detail + Spectrum, and Full report styles wil

Lab Exercise: Advanced Reporting Automated Spectral Library Search 130 Automated Spectral Library Search Make certain that Quant.M and Syss.D are st

Lab Exercise: Advanced Reporting Automated Spectral Library Search 131 to the calibration table for an identically named entry. If one is found, the

Lab Exercise: Introduction to the HPLC ChemStation Scheduler 6 Scheduler The Scheduler may be used to make your ChemStation automatically execute comm

Lab Exercise: Advanced Reporting Automated Spectral Library Search 132

Lab Exercise: Customized Report Design A Customized Report Layout view is included in the ChemStation for users who want to define the exact conte

Lab Exercise: Customized Report Design In this Laboratory You Will: 134 In this Laboratory You Will: • Build your own customized report template. •

Lab Exercise: Customized Report Design Preparations 135 Preparations 1) Enter the Report Layout view. Load the method Democal1.m (Double-click on t

Lab Exercise: Customized Report Design Building a Customized Report 136 Building a Customized Report 1) Under the File menu, select New Template. Fi

Lab Exercise: Customized Report Design Adding a General Section 137 Adding a General Section 1) Click on the blue triangle. The color will change t

Lab Exercise: Customized Report Design Inserting Tables 138 Inserting Tables 1) Below the chromatogram, expand the size of the general white space.

Lab Exercise: Customized Report Design Adding a Calibration Curve 139 Adding a Calibration Curve 1) Click on the blue triangle found between the Gen

Lab Exercise: Customized Report Design Finishing Up 140 Finishing Up 1) From the File menu, select Save Template As. Give the template a unique nam

Lab Exercise: Commands

Lab Exercise: Introduction to the HPLC ChemStation Scheduler 7 7) After you have witnessed the logbook display, return to the Scheduler to check the

Lab Exercise: Commands In this LaboratoryYou Will: 142 In this LaboratoryYou Will: • Become familiar with some of the more common commands on the H

Lab Exercise: Commands Retrieving Information about Commands 143 Retrieving Information about Commands Several resources are available to help you ut

Lab Exercise: Commands Tracking Errors and Using some Common Commands 144 Tracking Errors and Using some Common Commands A window can be displayed wh

Lab Exercise: Commands Registers, Objects, and Tables 145 Registers, Objects, and Tables A register is a place to store data such as chromatograms a

Lab Exercise: Commands Registers, Objects, and Tables 146 Objects Objects are the data you work with such as chromatograms or spectra. Each object

Lab Exercise: Commands Registers, Objects, and Tables 147 4) To integrate the chromatogram, use the command IntegrateObj. Type: IntegrateObj Chro

Lab Exercise: Commands Registers, Objects, and Tables 148 Try typing: Item$=ObjHdrName$(ChromReg[1],3) <enter> Obtype=ObjHdrType(ChromReg[1],i

Lab Exercise: Commands Registers, Objects, and Tables 149 Tables 1) You have already integrated the chromatogram with the IntegratObj command. Whe

Lab Exercise: Commands Registers, Objects, and Tables 150 to obtain a single numeric element from a table. Type: pk_width=tabval(ChromReg[1],"

Lab Exercise: Commands Registers, Objects, and Tables 151 Viewing Tables Often, you will need to display the contents of a table to understand what

Lab Exercise: Introduction to the HPLC ChemStation Maintaining the Windows 2000 Workstation 8 Maintaining the Windows 2000 Workstation (Skip to Maint

Lab Exercise: Commands Registers, Objects, and Tables 152

Lab Exercise: Macro Writing

Lab Exercise: Macro Writing In this Laboratory You Will: 154 In this Laboratory You Will: • Write and execute a macro. • Learn how to find errors

Lab Exercise: Macro Writing Writing and Executing a Macro 155 Writing and Executing a Macro During this first exercise, you will simply follow the di

Lab Exercise: Macro Writing Writing and Executing a Macro 156 Macro class.mac,go The macro should begin executing. If a problem is found, correct th

Lab Exercise: Macro Writing Write Your Own Macro 157 Write Your Own Macro Write a macro to do the following. Use your own registers and windows 2-1

Lab Exercise: Macro Writing Possible Answer 158 Possible Answer name overlaid file "c:\chem32\1\data\demo\005-0101.d" if regsize (myreg)

Appendix: Getting Started with New ChemStation Workflow G2170-90041

AAgilent ChemStation Getting Started with New ChemStation Workflow

Data OrganizationNotices© Agilent Technologies, Inc. 2006, 2007No part of this manual may be reproduced in any form or by any means (including elec-tr

Lab Exercise: Introduction to the HPLC ChemStation Maintaining the Windows 2000 Workstation 9 3) Click on the Tools tab. Find the Error-Checking too

Data OrganizationIn This Guide…In analytical laboratories, chromatography data need to be acquired efficiently in a short time. Clarifying ambiguous r

4 Data Organization

Data Organization 5Contents1 ChemStation Data Structure 7ChemStation prior to B.02.01 8ChemStation B.02.01/B.03.01 92 Data Acquisition 13Data Acquisit

6 Data OrganizationContents

7Agilent ChemStation Getting Started with New ChemStation WorkflowAgilent Technologies1ChemStation Data StructureChemStation prior to B.02.01 8ChemSta

8 Data Organization1 ChemStation Data StructureChemStation prior to B.02.01ChemStation prior to B.02.01 In ChemStation revisions prior to B.02.01, seq

Data Organization 9ChemStation Data Structure 1ChemStation B.02.01/B.03.01ChemStation B.02.01/B.03.01In order to strengthen the association between da

10 Data Organization1 ChemStation Data StructureChemStation B.02.01/B.03.01Similarly, the sequences in the folder Chem32\1\sequence serve as sequence

Data Organization 11ChemStation Data Structure 1ChemStation B.02.01/B.03.01The following chapters explain the impact of this structure on typical work

12 Data Organization1 ChemStation Data StructureChemStation B.02.01/B.03.01

Lab Exercise: Introduction to the HPLC ChemStation Maintaining the Windows XP Workstation 10 Maintaining the Windows XP Workstation (Skip this sectio

13Agilent ChemStation Getting Started with New ChemStation WorkflowAgilent Technologies2Data AcquisitionData Acquisition 14Data Acquisition in a Seque

14 Data Organization2 Data AcquisitionData AcquisitionData AcquisitionStarting with ChemStation B.02.01, flexible data storage for single runs and seq

Data Organization 15Data Acquisition 2Data AcquisitionData Acquisition in a Sequence In order to run a sequence, appropriate pre-defined methods must

16 Data Organization2 Data AcquisitionData AcquisitionThe sequence template references these methods in the sequence table.As explained previously, wh

Data Organization 17Data Acquisition 2Data Acquisitionthe container. All necessary files are now available for future data review and reprocessing, wi

18 Data Organization2 Data AcquisitionData Acquisition

19Agilent ChemStation Getting Started with New ChemStation WorkflowAgilent Technologies3Data AnalysisData Analysis 20Data Analysis: Data Review 23The

20 Data Organization3 Data AnalysisData AnalysisData Analysis Once the data have been acquired, they can be analyzed in ChemStation Data Analysis view

Data Organization 21Data Analysis 3Data AnalysisWith this toolset, you can jump to the beginning or end of the Navigation Table, step on to next or pr

22 Data Organization3 Data AnalysisData AnalysisFigure 8 Sequence Parameters in Method and Run Control view of ChemStation B.02.01 and higher

Lab Exercise: Introduction to the HPLC ChemStation Maintaining the Windows XP Workstation 11 3) Click on the Tools tab. Find the Error-Checking tool

Data Organization 23Data Analysis 3Data Analysis: Data ReviewData Analysis: Data ReviewData Review means analyzing on a run-per-run base. ChemStation

24 Data Organization3 Data AnalysisData Analysis: Data ReviewThe remainder of this section specifies which method is loaded during data review when a

Data Organization 25Data Analysis 3Data Analysis: Data ReviewYou load the master method, most conveniently from the Method tab of the ChemStation Expl

26 Data Organization3 Data AnalysisData Analysis: Data ReviewThis means that each time you load a run from the Navigation Table, the sequence method c

Data Organization 27Data Analysis 3Data Analysis: Data Reviewor last reprocessing. Additionally, it is possible to perform run-specific changes and sa

28 Data Organization3 Data AnalysisData Analysis: Data ReviewWhen the manual events have been saved to the individual method (instead of e.g. the corr

Data Organization 29Data Analysis 3The ChemStation User Interface during Data ReviewThe ChemStation User Interface during Data ReviewThe ChemStation u

30 Data Organization3 Data AnalysisThe ChemStation User Interface during Data ReviewThis feature is available in the following situations:It is import

Data Organization 31Data Analysis 3Data Analysis: Reprocessing dataData Analysis: Reprocessing dataIn contrast to data review, sequence reprocessing m

32 Data Organization3 Data AnalysisData Analysis: Reprocessing data• In the sequence table, it is not possible to add or remove lines.• In the Sequenc

Lab Exercise: Introduction to the HPLC ChemStation Maintaining the Windows XP Workstation 12 complete, you will be prompted for a blank, formatted flo

Data Organization 33Data Analysis 3Data Analysis: Reprocessing dataFigure 19 Sequence Parameters in Data Analysis

34 Data Organization3 Data AnalysisData Analysis: Reprocessing data

35Agilent ChemStation Getting Started with New ChemStation WorkflowAgilent Technologies4Workflow with Unique Folder Creation switched offThis chapter

36 Data Organization4 Workflow with Unique Folder Creation switched offWorking with Unique Folder Creation on or off?Working with Unique Folder Creati

Data Organization 37Workflow with Unique Folder Creation switched off 4Workflow with “Unique Folder Creation” switched offWorkflow with “Unique Folder

38 Data Organization4 Workflow with Unique Folder Creation switched offWorkflow with “Unique Folder Creation” switched offSelecting “Unique Folder Cre

Data Organization 39Workflow with Unique Folder Creation switched off 4Workflow with “Unique Folder Creation” switched off• There are no data file spe

40 Data Organization4 Workflow with Unique Folder Creation switched offWorkflow with “Unique Folder Creation” switched offThe enhanced ChemStation use

Data Organization 41Workflow with Unique Folder Creation switched off 4Workflow with “Unique Folder Creation” switched off• With the method usage opti

42 Data Organization4 Workflow with Unique Folder Creation switched offWorkflow with “Unique Folder Creation” switched off

Lab Exercise: Introduction to the HPLC ChemStation Windows Features (Windows 2000 or XP) 13 Windows Features (Windows 2000 or XP) Optional Section Mas

© Agilent Technologies 2006, 2007Printed in Germany 02/07*G2170-90041**G2170-90041*G2170-90041www.agilent.comAgilent TechnologiesIn This BookWith rev

Lab Exercise: Introduction to the HPLC ChemStation Windows Features (Windows 2000 or XP) 14 5) Check to make certain that you have copied the chosen

Lab Exercise: Introduction to the HPLC ChemStation Windows Features (Windows 2000 or XP) 15 Clipboard You may use the clipboard to capt

Lab Exercise: Introduction to the HPLC ChemStation Windows Features (Windows 2000 or XP) 16 If these actions do not clear your problem, you would have

Lab Exercise: Acquisition Methods

Lab Exercise: Acquisition Methods In this Laboratory You Will: 18 In this Laboratory You Will: • Prepare your instrument for acquisition. • Set-up

Lab Exercise: Acquisition Methods Preparation 19 Preparation Before starting, make certain that the you have the following: • A sample vial labele

Lab Exercise: Acquisition Methods Preparing the HPLC 20 Preparing the HPLC 1) Enter the Method and Run Control view of the Agilent Online ChemStati

Lab Exercise: Acquisition Methods Priming the Agilent 1100/1200 Solvent Delivery System 21 Priming the Agilent 1100/1200 Solvent Delivery System You

ChemStation B.03 Printed in April, 2007 Agilent 1100/1200 HPLC ChemStation Operation Course Number H4033A Laboratory Manual

Lab Exercise: Acquisition Methods Priming the Agilent 1100/1200 Solvent Delivery System 22 6) Wait until a steady stream of solvent co

Lab Exercise: Acquisition Methods Creating an Acquisition Method 23 Creating an Acquisition Method 1) From the Method menu select Edit Entire Meth

Lab Exercise: Acquisition Methods Practice Entering Gradients 24 Practice Entering Gradients 1) Select the Insert button from the Time Table group.

Lab Exercise: Acquisition Methods Practice Entering Gradients 25 Question: How is the threshold setting used? _________________

Lab Exercise: Acquisition Methods Practice Entering Gradients 26 8) Add DAD A to Selected Signals. Fill in an x-axis range of 10 minutes. Select t

Lab Exercise: Acquisition Methods Snapshot 27 Snapshot 1) Once the first major peak has eluted, open an Offline Session of the Agilent ChemStation.

Lab Exercise: Acquisition Methods Snapshot 28

Lab Exercise: Qualitative Data Analysis Ideally, use the data file generated from the isocratic test mix in the previous laboratory exercise.

Lab Exercise: Qualitative Data Analysis In this Laboratory You Will: 30 In this Laboratory You Will: 1) Use signal options to display data. 2) Ann

Lab Exercise: Qualitative Data Analysis Graphics - Signal Options 31 Graphics - Signal Options 1) Enter the Data Analysis view. 2) Load the method

ii Notice The information contained in this document is subject to change without notice. Agilent Technologies makes no warranty of any kind with rega

Lab Exercise: Qualitative Data Analysis Graphics - Signal Options 32 11) A panel with 3 tabs will appear. The Signals tab should be highlighted and

Lab Exercise: Qualitative Data Analysis Graphics - Annotation 33 Graphics - Annotation 1) Using the Signal(s) Displayed drop box, select DAD1A only

Lab Exercise: Qualitative Data Analysis Graphics - Annotation 34 7) To print this window, pull down the File menu and select Print, Selected Window.

Lab Exercise: Qualitative Data Analysis Window Functions 35 Window Functions 1) Use the Signal(s) Displayed drop box to view All Loaded Signals. 2)

Lab Exercise: Qualitative Data Analysis Peak Purity 36 Peak Purity The Peak Purity algorithm can help you determine whether a chromatographic peak i

Lab Exercise: Qualitative Data Analysis Peak Purity 37 Major Peaks Peak 1 Peak 2 Peak 3 Retention Time Calculated Threshold Purity Facto

Lab Exercise: Qualitative Data Analysis Automated Peak Purity 38 Automated Peak Purity The Short + Spectrum, Detail + Spectrum, and Full report styl

Lab Exercise: Qualitative Data Analysis Displaying Spectra 39 Displaying Spectra 1) With demo\demodad.d still loaded, use the signals drop box to s

Lab Exercise: Qualitative Data Analysis Isoabsorbance Plot 40 Isoabsorbance Plot In this section, you will try to optimize the data acquisition samp

Lab Exercise: Qualitative Data Analysis Isoabsorbance Plot 41 selected in Data Analysis, that of the peak at 4.85 minutes. Convince yourself that th

iii Table Of Contents LAB EXERCISE: INTRODUCTION TO THE HPLC CHEMSTATION... 1 IN THIS LABORATORY YOU WILL: ...

Lab Exercise: Qualitative Data Analysis 3D Plot 42 3D Plot 1) From the Spectra menu, select 3D Plot. Maximize the window. 2) Rotate the 3D plot.

Lab Exercise: Spectral Libraries

Lab Exercise: Spectral Libraries In this Laboratory You Will: 44 In this Laboratory You Will: • Build a spectral library. • Perform a library searc

Lab Exercise: Spectral Libraries Building a Library 45 Building a Library 1) From the View menu, select Data Analysis. 2) Load Class.D created in

Lab Exercise: Spectral Libraries Building a Library 46 11) Click on Name and type in Dimethylphthalate. (Do not press Enter, as doing so will comple

Lab Exercise: Spectral Libraries Library Searching 47 Library Searching In this section you will test your library with the same data file. Later we

Lab Exercise: Spectral Libraries Library Searching 48

Lab Exercise: Overview and Diagnostics

Lab Exercise: Overview and Diagnostics In this Laboratory You Will: 50 In this Laboratory You Will: • Learn to correctly identify the hardware eleme

Lab Exercise: Overview and Diagnostics The Agilent 1200 Flow Path 51 The Agilent 1200 Flow Path Please locate the items listed below with your instruc

iv LAB EXERCISE: SPECTRAL LIBRARIES ... 43 IN THIS LABORATORY YOU WILL: ...

Lab Exercise: Overview and Diagnostics Error Messages and the Logbook 52 Error Messages and the Logbook When an error (blockage, component failure, et

Lab Exercise: Overview and Diagnostics Diagnosis View 53 Diagnosis View The 1200 Series ChemStation is equipped with a view to help you: 1) diagnose s

Lab Exercise: Overview and Diagnostics Diagnosis View 54 Note: Exceeding an EMF limit will not shut down your system or abort any method or sequence.

Lab Exercise: Overview and Diagnostics Diagnosis View 55 Maintenance Logbook, and Date Changes Logbook. Examine each one of these entries. 5) Click

Lab Exercise: Overview and Diagnostics Diagnosis View 56 7) Click on the Start button located next to the Leak Test option. The Leak Test is display

Lab Exercise: Overview and Diagnostics Diagnosis View 57 maintenance CD-ROM and then learn to perform user maintenance procedures on the 1200 modules.

Lab Exercise: Overview and Diagnostics Diagnosis View 58 Other important information found on the Maintenance and Repair CD includes Part and Material

Lab Exercise: Overview and Diagnostics Routine Maintenance 59 Routine Maintenance Using the CD-ROM, perform the instrument maintenance listed below.

Lab Exercise: Overview and Diagnostics Instrument Tests 60 Instrument Tests The tests have been saved for the end of the laboratory in order to make

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series

v LAB EXERCISE: RUNNING A SEQUENCE... 99 IN THIS LABORATORY YOU WILL: ...

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series In this Laboratory You Will: 62 In this Laboratory You Will: • Learn to correctly i

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series The Agilent 1200 - SL Flow Path 63 The Agilent 1200 - SL Flow Path Please locate t

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Error Messages and the Logbook 64 Error Messages and the Logbook When an error (bloc

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Agilent LC Diagnostic Tool 65 Agilent LC Diagnostic Tool The Agilent 1200 Series H

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Agilent LC Diagnostic Tool 66 this case you may want to set the flow rate to 2-3 mL/m

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Agilent LC Diagnostic Tool 67 2) The Test Selection panel is displayed. Select th

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Agilent LC Diagnostic Tool 68 part of the repair procedure. For practice, select the

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Instrument Status Report 69 Instrument Status Report You can print a status report fo

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Routine Maintenance 70 Routine Maintenance Using the CD-ROM, perform the instrument

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Instrument Tests 71 Instrument Tests The tests have been saved for the end of the la

vi

Lab Exercise: Overview and Diagnostics for the Agilent-SL Series Instrument Tests 72

Lab Exercise: Integration

Lab Exercise: Integration In this Laboratory You Will: 74 In this Laboratory You Will: • Perform autointegration. • Integrate using the Events Tab

Lab Exercise: Integration Integration Preparation 75 Integration Preparation ChemStation revisions B.01 and higher have a new, more efficient and ac

Lab Exercise: Integration Auto Integration 76 Auto Integration One of the easiest ways to obtain initial integration events is through Auto Integra

Lab Exercise: Integration Auto Integration 77 5) Using the Events Table drop box, select signal DAD1 B Specific. 6) To Auto Integrate, select the

Lab Exercise: Integration Integrate Using the Events Table 78 Integrate Using the Events Table Now, you will set up integration events for the Integ

Lab Exercise: Integration Adding Timed Events 79 Adding Timed Events At times, the initial events are not able to properly construct baselines or pl

Lab Exercise: Integration Adding Timed Events 80 6) Practice adding other timed events. Note, you can also add and remove timed events with the tool

Lab Exercise: Integration Using Manual Events 81 Using Manual Events Manual integration allows you to integrate specific peaks or groups of peaks wi

Lab Exercise: Introduction to the HPLC ChemStation

Lab Exercise: Integration Using Manual Events 82 1) Select Copy Manual Events to Method in the Integration menu. 2) Select the Edit/Set Integration

Lab Exercise: Quantification

Lab Exercise: Quantification In this Laboratory You Will: 84 In this Laboratory You Will: • Run three external standards to create a calibration ta

Lab Exercise: Quantification Preparations 85 Preparations Obtain the low, medium and high standards. Insert them into vial positions 1, 2 and 3 of

Lab Exercise: Quantification Preparations 86 5) To turn the solvent delivery system on, you may use the menu items: Instrument, More Pump, Control;

Lab Exercise: Quantification Creating the Acquisition Method 87 Creating the Acquisition Method 1) Load the default method, DEF_LC.M as a starting

Lab Exercise: Quantification Creating the Acquisition Method 88 Note: If a VWD detector is being utilized, select the same wavelength as signal A.

Lab Exercise: Quantification Data Acquisition-Standards 89 Data Acquisition-Standards In this example, you will run each sample individually, not as

Lab Exercise: Quantification Data Acquisition-Standards 90 2) Check that your baseline and pressure are stable. 3) Start the run either from the S

Lab Exercise: Quantification Integration 91 Integration In this section you will optimize the integration events for the analysis and save them to th